Blood Group Genotyping

ReadyPlex™ – Rapid genotyping for blood grouping, Platelet (HPA), Neutrophils (HNA) and coagulation factors

(Currently in development and looking for commercialisation partners)

 

Real-time PCR blood group genotyping

The Biofortuna ReadyPlex™ product family is an exciting new range of real-time PCR-based products for genomic analysis of erythrocyte blood groups, Human Platelet Antigens (HPA), Human Neutrophil Antigens (HNA) and coagulation polymorphisms. The kits are ready to go – just add DNA to the ReadyPlex™ assay and amplify. After PCR, the ReadyPlex Clarity™ software enables easy genotyping.

 

HemoPlex™ RBC genotyping

A series of modular kits that can be used in combination to define a range of blood group genotypes, including the commonly requested RHD, RHCE, Kell, Kidd and Duffy polymorphisms.

Product NumberProduct NameSpecificationFormatTests per Kit
HP1HemoPlex RHD Screening KitDetermination of D+/- status: RHD exons 1, 2, 3, 4 & 7 and detection of RHD psi allele8 Reactions60
HP2HemoPlex RHCE Screening KitRHCE C, c, E, e, Cw,VS+/-8 Reactions60
HP4HemoPlex RHD & CE Genotyping Kit Genotyping both RHD and RHCE exons 1-10 identifying multiple alleles groups, variants and RHD CE recombinant variants48 Reactions10
HP6HemoPlex RHCE Variant Genotyping KitRHCE C, c, E, e, Cw, Cx, V+/-, VS+/-, (C)ces and various variants24 Reactions20
HP7HemoPlex Kell Genotyping KitK, k, Kw, Kpa, Kpb, Kp c, Jsa, Jsb8 Reactions60
HP8HemoPlex Kidd & Duffy Genotyping KitFya, Fyb, Fya-b, Fyx, Jka, Jsb8 Reactions60
HP10HemoPlex MNS Genotyping KitM, N, S, s, U, U-8 Reactions60
HP11HemoPlex RHD Variant Genotyping KitDetermination of D+/- status: RHD exons 1 to 10 and detection of RHD psi allele, D weak types 1-6 & 15, various partial RHD24 Reactions20
HP27HemoPlex Multi Blood Group Genotyping KitCartwright, Colton, Cromer, Diego, Dombrock, Duffy, Indian, Kidd, Knops, LW, Lutheran, OK, Scianna, Vel32 Reactions15

 

ThromboPlex™ platelet genotyping

A set of modular kits that can be used for genotyping common HPA-1 to 5, 12 and 15 mutations, or a comprehensive test for all 29 HPA alleles.

Product NumberProduct NameSpecificationFormatTests per Kit
TP1ThromboPlex HPA Screening Kit HPA-1a/b, 2a/b, 3a/b, 4a/b, 5a/b, 12aw/bw, & 15a/b 8 Reactions60
TP3ThromboPlex HPA Genotyping Kit HPA-1a/b to 29bw 32 Reactions15

 

NeutroPlex™ neutrophil genotyping

Kits for HNA-1, 3, 4 and 5 screening.

Product NumberProduct NameSpecificationFormatTests per Kit
NP1NeutroPlex HNA Genotyping KitDefines A & B alleles of HNA1, 3, 4, 58 Reactions60

 

CoaguPlex™ coagulation factor genotyping

Kits for Factor V Leiden, Factor II G20210A and MTHFR (methylenetetrahydrofolate reductase) C677T.

Product NumberProduct NameSpecificationFormatTests per Kit
CG1CoaguPlex Genotyping Kit 1691G>A Factor V Leiden polymorphism,
prothrombin (factor II) 20210G>A, MTHFR C677T
8 Reactions60

 

Please enquire for other blood group antigens, kit combinations or polymorphisms

For Investigational Use Only. Products currently in development.

 

ReadyPlex workflow 

Biofortuna’s ReadyPlex kits analyze genomic DNA. Each PCR reaction has an internal positive control to verify successful amplification allowing confident allelic typing.

Readyplex easy image 2